Figure 5. The deletion of LuxS gene decreased the biofilm formation ability of E. faecalis. (a) Confocal laser scanning microscope images of biofilm formation on Δ LuxS E. faecalis strain at 12 h, 24 h, 36 h and 48 h. Scale bar=50 μm; (b) The biofilm areas were measured using an image-processing program; (c) Quantifying the biofilm by crystal violet staining, One-way ANOVA was used to analyze the statistical significance. All error bars signify standard deviation (n=3). *:P<0.05.