Figure 4.

TRIM44 promotes human esophageal cancer (HEC) cell migration and invasion by promoting epithelial mesenchymal transition (EMT) and histopathological correlation of TRIM44 expression and EMT phenotypes in the tissue. A,B, Effects of TRIM44 overexpression and down‐regulation on epithelial and mesenchymal makers (including E‐cadherin, Vimentin and N‐cadherin) using fluorescence staining; C,D, changes in key molecules of EMT determined by western blot analyses after overexpression and down‐regulation of TRIM44 in KYSE140 and KYSE510 cells, respectively. E, High TRIM44 expression was associated with impaired E‐cadherin expression and high levels of Vimentin. Low TRIM44 expression was associated with increased E‐cadherin expression and low levels of Vimentin. F, The correction between TRIM44 and E‐cadherin expression according to the integrated optical density of the immunostaining. B, The correction between TRIM44 and Vimentin expression according to the integrated optical density of the immunostaining