Figure 1.

Knockdown of Ambra1 increased epirubicin (EPI)‐induced apoptosis. A, The basal expression of Ambra1 in MCF‐7, MDA‐MB‐231 and SK‐Br‐3 cells was detected by western blotting. B, Cells were incubated with scrambled shRNA or target‐specific AMBRA1 shRNA (2450 or 3388) for 48 h, the protein of Ambra1 was examined by western blotting, and the mRNA was examined by quantitative RT‐PCR (C). The results (mean ± SE) are from 3 independent experiments (*P < 0.05). D, Cells were transfected with scrambled shRNA or 2450 or 3388 for 48 h, following treatment with 2.2 μmol/L EPI for another 24 h; then, cell viability and mortality were analyzed. At the same time, caspase‐9 activity and apoptosis were measured (E). The results (mean ± SE) are from 3 independent experiments (*P < 0.05)