Figure 3.

Ambra1 regulated epirubicin (EPI)‐induced autophagy in breast cancer cells. A, MDA‐MB‐231 cells were incubated with scrambled shRNA or target‐specific AMBRA1 shRNA (2450 or 3388) for 48 h. The protein of Ambra1 was detected by western blotting. B, MDA‐MB‐231 cells were incubated with scrambled shRNA or 2450 or 3388 for 48 h, followed by treatment with EPI for another 24 h in the presence or absence of Bafilomycin A1 (BAF1, 20 nmol/L); then, the protein of LC3‐I/II and p62 was detected by western blotting. C, MDA‐MB‐231 cells expressing RFP‐GFP‐LC3 were incubated with scrambled shRNA or 2450 or 3388 for 48 h. Then, the cells were treated with EPI for another 24 h in the presence or absence of Bafilomycin A1 (BAF1, 20 nmol/L). Autophagy was assessed with the LC3 puncta. The results (mean ± SE) are from 3 independent experiments (*P < 0.05)