Figure 2.

Knockdown of Tat‐interacting protein 30 (TIP30) promoted lung cancer cell epithelial‐to‐mesenchymal transition (EMT), invasion and stemness. (A) The bright‐field images of the scrambled and TIP30‐knockdown stable clones of A549 and PC9 cells. Scale bar = 20 μm. (B) The expression of the indicated proteins in the scrambled and TIP30 knockdown stable clones of A549 and PC9 cells was examined by western blotting. (C) The in vitro invasive ability of the scrambled and TIP30 knockdown stable clones of A549 and PC9 cells was examined by a transwell invasion assay. A quantitative analysis of the invading cells was performed. *P < .05. (D) The mRNA levels of the stemness‐related genes in the scrambled and TIP30 knockdown stable clones of A549 and PC9 cells were examined by quantitative PCR. *P < .05. € The sphere formation ability of the scrambled and TIP30‐knockdown stable clones of A549 and PC9 cells was examined. *P < .05