Figure 1.

Dysregulation of HOMEOBOX A11 antisense RNA (HOXA11‐AS) is associated with cisplatin resistance of lung adenocarcinoma (LUAD) cells. (A) The expression level of HOXA11‐AS in LUAD tissues and non‐tumorous tissues of The Cancer Genome Atlas (TCGA) database was analyzed and shown. (B) Based on TCGA dataset, a survival curve was generated to analyze the correlation between HOXA11‐AS expression and the overall survival of LUAD patients. (C) Quantitative RT‐PCR was used for detection of the expression level of HOXA11‐AS in parental cells (A549 and H157) and cisplatin‐resistant cells (A549‐CR and H157‐CR). (D) The 50% inhibitory concentration (IC₅₀) value of parental cells and corresponding cisplatin‐resistant cells was tested with MTT assay. (E) HOXA11‐AS was overexpressed in A549 and H157 cells by transfecting with pLent‐HOXA11‐AS. (F) HOXA11‐AS was downregulated in cisplatin‐resistant cells by transfecting with short hairpin RNA (sh)‐HOXA11‐AS. (G) The IC₅₀ value of parental cells was examined after HOXA11‐AS was overexpressed. (H) The IC₅₀ value of cisplatin‐resistant LUAD cells was detected after HOXA11‐AS was knocked down. Error bars represent the mean ± SD of at least three independent experiments. *P < .05, **P < .01, ***P < .001 vs control group