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. 2018 Sep 2;109(10):3068–3079. doi: 10.1111/cas.13764

Figure 5.

Figure 5

HOMEOBOX A11 antisense RNA (HOXA11‐AS) is a sponge of miR‐454‐3p. (A) Subcellular fractionation assays revealed that HOXA11‐AS was mainly located in cytoplasm. (B) RNA immunoprecipitation (RIP) assays were performed in A549‐CR and H157‐CR cells by using anti‐Ago2. (C) The binding sites between wild type HOXA11‐AS (HOXA11‐AS‐WT) or mutant type HOXA11‐AS (HOXA11‐AS‐MUT) and miR‐454‐3p were predicted with bioinformatics analysis. (D) Dual luciferase reporter assay was applied to demonstrate the combination between HOXA11‐AS and miR‐454‐3p in A549‐CR and H157‐CR cells. (E) The higher expression level of miR‐454‐3p was tested in parental cells by using quantitative RT‐PCR. (F) The expression level of HOXA11‐AS was examined in A549‐CR and H157‐CR cells in response to miR‐454‐3p mimics. (G) The expression level of HOXA11‐AS was examined in parental cells in response to miR‐454‐3p inhibitor. Error bars represent the mean ± SD of at least three independent experiments. **P < .01 vs control group