The mRNA expression levels of CSF1, BDNF, NGF, VEGF, GDNF, and TGFβ1 in the spinal dorsal horn (A, B) and thalamus (C, D) were evaluated with RT-qPCR using the comparative CT method (2−ΔCT). The basal mRNA expression levels of these growth factors relative to β-actin (ACTB) in the non-diabetic group are shown in A and C. Fold changes of mRNA expression levels in the type 2 diabetic group compared to those in the non-diabetic control group are shown in B and D. Each value is presented as the mean ± SEM (n=6–7).