Table 1.
Primer sequences for RT-qPCR
| Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
| ACTB | TCTTCCAACCTTCCTTCCTG | TGTGTTGGCGTACAGGTCTT |
| IL-1β | GCACCTTGATTCCCTTCATC | TGCAGTGCAGTGATCGTACA |
| IL-6 | TCAGCCCTGAGAAAGGAGAC | TTTCAGCCATCTTTGGAAGG |
| IL-18 | ATCGGCCCCTATTTGAAGAT | CCATACCTCTAGGCTGGCTAT |
| TNFα | TGTCTGCTGCACTTTGGAGT | CTTGGGGTTCGAGAAGATGA |
| IFNγ | GTCCAACGCAAAGCAGTACA | TCGACCTCGAAACATCTGAC |
| IL-1RN | GGAAGATGTGCCTGTCCTGT | AGCGCTTGTCCTGTTTTCTG |
| IL-4 | AACTGCCATATCGCCTTACG | GCAGCAAGGATGTCCGTTAT |
| IL-10 | GCGCTGTCATCGATTTCTTC | TGGCTTTGTAGACGCCTTTC |
| IL-13 | ATGGTGTGGAGCATCAACCT | TTCAGCATCCTCTGGGTCTT |
| IL-18BP | ACTGAATGGAACGCTGACCT | GGAGGTGCTCAATGAAGGAA |
| TLR1 | TTCTGGGGTTGAGCACTACA | TTGTCCCCATAATGCTCTCC |
| TLR2 | GCCTCTCCAAGGAAGAATCC | GAGCACTGAGGGAATGGAGT |
| TLR3 | TTGCTTGGCTTCCACAACTA | TCAGGTACCGCACATTGAAA |
| TLR4 | AATCCCCTGAGGCATTTAGG | CCCCATCTTCAATTGTCTGG |
| TLR5 | GACAAGGAGGCCTTCAGAAA | GGAACAGTCCCTGAAAAGCA |
| TLR6 | CGCAGACAGCATTGTACACC | GAATGTGCTTGGTGCATGAG |
| TLR7 | TAAGTGGAAATTGCCCTCGT | TTCTGTCAGCGCATCAAAAG |
| TLR8 | CTGAGCAACACCCAGATCAA | ATTGAAGCACCTCGGACAAT |
| TLR9 | CACAAGAGGGTGTCCTTTGC | ATCGAGTGAGCGGAAGAAGA |
| TLR10 | TTCTGTCTTCAGGTGCTTGC | AGCTCTCGTAAGGCCATCAG |
| CSF1 | TCAGAGACAACACCCCCAAT | GGCCTTGTCATGCTCTTCAT |
| BDNF | CAAACATCCGAGGACAAGGT | GATGTCAGGCCTCTTGAACC |
| NGF | GGGAGCAGCTTTCTATCCTG | GTGTGGTTCTGCCTGTATGC |
| VEGF | TCTTCAAGCCATCCTGTGTG | CTGCATGGTGATGTTGGACT |
| GDNF | CCGGGGTTGTGTCTTAACTG | AGAGCCGCTGCAGTACCTAA |
| TGFβ1 | CAGCTCCACGGAGAAGAACT | CAGAAGTTGGCATGGTAGCC |
| CB1 | ATCCTAGATGGCCTTGCAGA | TGCCATGTCACCTTTGATGT |
| CB2 | TCCACTGATTCCCAACGACT | TGATGGGCTTTCCAGAGAAC |