Fig. 10.

Correlation between EMP1 expression and malignancy of human prostate cancer. a Representative H–E staining of human prostate cancer samples with lower Gleason score (GS; 6 or 7) or higher Gleason score (8 or 9). Arrowheads: accumulation of cancer cells; Arrows: invasive cell front. b, d Representative results of immunohistochemistry for EMP1 and P-Src expression. Nuclei were counterstained with hematoxylin. Arrowheads: EMP1- or P-Src-positive cancer cells. c, e Summary graph of H-score. **p < 0.01 vs. lower Gleason score. f Summary graph of qPCR results in samples with lower or higher Gleason score. Relative mRNA expression of EMP1 was normalized to that of β-actin. **p < 0.01 vs. lower Gleason score. g Schematic illustration of EMP1-induced signal transduction in cancer cells. Scale bars, 100 μm in a and 50 μm in b, d