Fig. 5.

Effect of copine-III on EMP1 induced increase in cell migration. a Knockdown of copine-III. Two siRNAs (D3 and D5) for copine-III were applied to LNCaP #2 and #17 cells, and the cell lysates were used for western blotting. GAPDH: loading control. b Representative results of the Boyden chamber assay. Scale bar, 200 μm. c Summary graph of migrated LNCaP cells in the Boyden chamber assay. *p < 0.05 and **p < 0.01. d Expression levels of endogenous copine-III and transfected EGFP, EGFP-copine-III-WT or -ΔVWA in copine-III-knockdown LNCaP #17 cells. e Summary graph of migrated LNCaP #17 cells after knockdown of copine-III with or without re-expression of EGFP-copine-III-WT or -ΔVWA in the Boyden chamber assay. **p < 0.01. f Summary graph of migrated LNCaP #2 and #17 cells transfected with EGFP or EGFP-copine-III-WT in the Boyden chamber assay