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. 2018 Jun 4;37(40):5416–5434. doi: 10.1038/s41388-018-0286-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — EMP1 induced activation of Rac1. a, c GTP-bound active form of Rac1 in each type of LNCaP cells was pulled down using beads bound to GST-PAK-CRIB, followed by western blotting. b, d Summary graph of the relative density ratio of GTP-bound Rac1 normalized to total Rac1. The value of the relative density ratio in LNCaP #2 (b) and scramble RNA- and EGFP-transfected LNCaP #17 (d) was set at 1.0. **p < 0.01 vs. LNCaP #2 in b. **p < 0.01 in d