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. 2018 Oct 4;8:14828. doi: 10.1038/s41598-018-33234-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DMSO treatment affects protein secondary structures in HCT-116 cells. (A) The representative second derivative and vector-normalized IR spectra of HCT-116 cells in Amide I (1680–1600 cm⁻¹) spectral region demonstrating protein secondary structure bands. (B) The band intensities (%) of total α-helix and β-sheet structures in DMSO treated and untreated (UT) HCT-116 cells.