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. 2018 Oct 4;8:14828. doi: 10.1038/s41598-018-33234-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DMSO treatment influences cellular lipid content and status in HCT-116 and SW-480 cells. (A) The band intensities (%) and wavenumber values of cholesteryl esters in DMSO treated and untreated (UT) HCT-116 and, (B) in SW-480 cells. (C) The intensities (%) of olefinic and olefinic/total lipids bands in DMSO treated and UT HCT-116 and, (D) SW-480 cells. The results are represented as mean ± SEM. t test was used to compare the results with respect to untreated cells. (E) Left Panel: PCA 3D scores plots for HCT-116 and SW-480 cells in 3030–2830 cm⁻¹ and 4000–650 cm⁻¹ spectral regions. Right Panel: LDA discrimination plots, and prediction and confusion matrices for HCT-116 and SW-480 cells in 3030–2830 cm⁻¹ and 4000–650 cm⁻¹ spectral regions.