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. 2018 Oct 4;8:14822. doi: 10.1038/s41598-018-32917-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expected and detected sequence counts (as percentages) of target specimens (at the level of phyla) in the artificial sample for cytochrome c oxidase subunit I (COI) and nuclear hypervariable region V8 of 18S rDNA samples. Percentages are calculated by taxon reads to all identified reads per gene region. Expected reads calculated based on equalized ngDNA amount, with taxa excluded for 18S rDNA which cannot be sequenced in the 125-bp paired-end reading length. Prepared by Babett Günther using Microsoft Excel 2010 (www.microsoft.com).