Table 1.
PCR primers, including their tags for sample pooling.
| Primer name | Direc-tion | Sequence 5′-3 | Reference |
|---|---|---|---|
| COI | |||
| mlCOIintR | R | GGRGGRTASACSGTTCASCCSGTSCC | Leray et al.31 |
| mlCOIintK | R | GGRGGRTAWACWGTTCAWCCWGTWCC | Present study |
| nsCOIFo | F | THATRATNGGNGGNTTYGGNAAHTG | Present study |
| Tags | F | actgac, gtagca, gacagt, tgtacg, catctg | Present study |
| Tags | R | gacgta, tctgag, gctagt, agcact, agagac | Present study |
| 18S | |||
| 18S#4 | F | AGGTCWGTRATGCCCTYMG | Machida & Knowlton 201229 |
| 18S#5_RC | R | TGYACAAAGGBCAGGGAC C | Machida & Knowlton 201229 |
| Tags | F | gtca, catg, acgt, tagc, agac | Present study |
| Tags | R | actc, gaga, ctct, gcat, tgag | Present study |