Table 3.
Specific activities of PmMOR, PmMOR mutants and Dl5ß–POR with progesterone, 5α-dihydroprogesterone, 5β-dihydroprogesterone and citral.
| Protein | Specific activity (pmol/min/μg) | Substrate |
|---|---|---|
| PmMOR | 3.07 ± 0.38 | progesterone |
| PmMOR | 2.81 ± 0.12 | 5α-dihydroprogesterone |
| PmMOR | 2.56 ± 0.23 | 5β-dihydroprogesterone |
| Dl5ß–POR | 1.79 ± 0.24 | progesterone |
| Dl5ß–POR | no activity | 5α-dihydroprogesterone |
| Dl5ß–POR | no activity | 5β-dihydroprogesterone |
| Dl5ß–POR | 27.5 ± 2.86 | citral |
| PmMOR A346V/I350N | 2.30 ± 0.18 | progesterone |
| PmMOR V150M/I156Y | 7.80 ± 0.97 | progesterone |
| PmMOR V150M | 7.67 ± 0.47 | progesterone |
| PmMOR | 760 ± 14 | citral |
| PmMOR V150M | 883 ± 5.7 | citral |
| PmMOR N205D | 5400 ± 5.94 | citral |
| PmMOR V150M/I156Y | 1694 ± 52.5 | citral |