Skip to main content
. 2018 Sep 28;12:328. doi: 10.3389/fncel.2018.00328

Figure 2.

Figure 2

Morphological characterization of Gad67-GFP positive hSOD1G93A cortical interneurons in vitro. (A) Representative images of patched interneurons reconstructed from post hoc neurobiotin-streptavidin labeling in Gad67-GFP::hSOD1G93A and Gad67-GFP::WT cultures. Each concentric circle represents 10 μm, and each dashed line represents 50 μm from the cell soma. (B) Sholl analysis denoting the morphological complexity of GFP-positive interneurons as measured by the average number of neurites intersecting with concentric circles placed at 10 μm intervals from the cell soma (Gad67-GFP::hSOD1G93A, n = 11 cells from five cultures; Gad67-GFP::WT, n = 20 cells from five cultures; *p < 0.05, two-way ANOVA), error bars show mean ± SEM. (C–E) Histograms quantifying significantly increased total neurite path length (μm; C), total branch number (D) and average number of branches (E) of Gad67-GFP::hSOD1G93A interneurons compared to Gad67-GFP::WT controls (Gad67-GFP::hSOD1G93A, n = 11 cells from 5 cultures; Gad67-GFP::WT, n = 20 cells from five cultures; *p < 0.05, Mann-Whitney test). Box-and-whisker plots show the interquartile range.