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Strategy for generating different chimeras from α-amylase of B. acidiola. In the first step, amylase was engineered by adding 11 and 37 amino acids to N- and C- terminal ends from the α-amylase of G. thermoleovorans. In the second attempt, the engineered amylase and glucoamylase (from Aspergillus niger) were fused through a linker peptide of 25 amino acids [(Gly-Gly-Thr-Gly-Ser)5] {(Gly-Gly-Thr-Gly-Ser)5} (modified with permission from Parashar and Satyanarayana, 2016c, 2017a).
