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. 2018 Sep 14;18(5):4523–4529. doi: 10.3892/mmr.2018.9484

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — CVF pretreatment reduces the release of inflammatory mediators. Inflammatory mediators (A) TNF-α and (B) IL-1β, and (C) anaphylatoxin C5a were assessed using ELISAs at different times following IRI initiation. (D) Expression of anaphylatoxin C5a protein was evaluated by western blotting analysis in liver tissues at different times after IRI initiation, and the protein levels were calculated relative to GAPDH. IRI, ischemia-reperfusion injury; CVF, cobra venom factor; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β.