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. 2018 Sep 14;18(5):4428–4438. doi: 10.3892/mmr.2018.9485

Figure 5.

Figure 5.

FKBP11 is involved in IFN-γ/TNF-α-induced apoptosis in HT-29 cells. (A) HT-29 cells were transfected with either the FKBP11 overexpressing plasmid pCMV-HA-FKBP11, blank control plasmid pCMV-HA, controls siRNA or FKBP11siRNA for 48 h and then treated with IFN-γ (2.5 ng/ml) and TNF-α (50 ng/ml) for 24 h. Western blot analyses detected FKBP11, BAX and active caspase-3 expression levels following treatment with IFN-γ/TNF-α compared with the untreated control and control plasmid (HA). #P<0.05 vs. IFN-γ/TNF-α untreated control; $P<0.05 vs. control plasmid pCMV-HA. (B) Western blot analyses detected FKBP11, BAX, active caspase-3 and PCNA following transfection with FKBP11 siRNA and treatment with IFN-γ (2.5 ng/ml) and TNF-α (50 ng/ml) for 24 h. Data are presented as mean ± standard error (n=3). *P<0.05 vs. control siRNA. HA, hemagglutinin; FKBP11, FK506 binding protein 11; siRNA, small interfering RNA; GRP78, 78 kDa glucose-regulated protein; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; BAX, Bcl2 associated X apoptosis regulator; PCNA, proliferating cell nuclear antigen.