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. 2018 Sep 14;18(5):4321–4327. doi: 10.3892/mmr.2018.9483

Figure 4.

Figure 4.

Western blotting of cleaved caspase-3, p53 and Bcl-2 in the three cell lines. Western blotting of (A) the human pulmonary type II-like epithelial lung adenocarcinoma cell line A549, (B) the human umbilical vein endothelial cell line CRL-1730, and (C) the human bladder carcinoma cell line ECV304. A, Untreated control group (no LPS, no AM); b, LPS group (1 µg/ml), and the four LPS+AM groups treated with LPS (1 µg/ml); plus c, 25; d, 50; e, 100 and f, 200 µg/ml of AM. β-actin served as a loading control. Data are presented as the mean ± standard deviations from three independent experiments. #P<0.05 vs. control group a and *P<0.05 vs. LPS group b with one-way analysis of variance followed by Bonferroni post-hoc test. AM, Astragalus membranaceus; LPS, lipopolysaccharide; Bcl-2, B cell lymphoma-2.