Table I.
Gene name | Sense primer | Anti-sense primer |
---|---|---|
p53 | 5′-TAACAGTTCCTGCATGGGCGGC-3′ | 5′-AGGACAGGCACAAACACGCACC-3′ |
Caspase-3 | 5′-TTAATAAAGGTATCCATGGAGAACACT-3′ | 5′-TTAGTGATAAAAATAGAGTTCTTTTGTGAG-3′ |
Bax | 5′-TGGAGCTGCAGAGGATGATTG-3′ | 5′-GAAGTTGCCGTCAGAAAACATG-3′ |
GAPDH | 5′-TCCCTCAAGATTGTCAGCAA-3′ | 5′-AGATCCACAACGGATACATT-3′ |
Bcl-2 | 5′-CACCCCTGGCATCTTCTCCTT-3′ | 5′-AGCGTCTTCAGAGACAGCCAG-3′ |
Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.