Table I.
Exon | Forward (5′-3′) | Reverse (5′-3′) | Product size (bp) | Temperature (°C) |
---|---|---|---|---|
4 | TGCAGCTGCCCGAGGATTA | GCACCCCGAGCCCGAAGTC | 144 | 65 |
5 | TCCCTCTTCTTCCTCTTCACT | GGGGTCCATAATTAGCATC | 301 | 58 |
5a,6 | GCTCTCTACAGTAAGTTCTC | AGGAGAGAGCATTGGGCTTA | 457 | 59 |
7 | AATCCACCCACTGTCCCG | CCAGCCACCTTCATACCG | 542 | 59 |
8 | TCAGGTAACTAACATCGCA | GTTGACTGTACTTGGAAGAA | 719 | 53 |
9,10,11 | GAGGTGGGAACCAGTTTGATG | CAAGCCAATCTCTGTAGTGCG | 890 | 55 |
12 | GCTGTGTGATGTGTTCCTCA | AAGAGAGATCGCCTCTGTG | 245 | 58 |
13 | CATGTCTGTTTCTCAAAGGG | CCATAGTCACTGACTGAATTAACAC | 202 | 59 |
PAX 6, Paired Box 6.