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. 2018 Sep 10;18(5):4439–4445. doi: 10.3892/mmr.2018.9469

Table I.

Summary of the primers and products size used for the amplification of the PAX6 exons.

Exon Forward (5′-3′) Reverse (5′-3′) Product size (bp) Temperature (°C)
4 TGCAGCTGCCCGAGGATTA GCACCCCGAGCCCGAAGTC 144 65
5 TCCCTCTTCTTCCTCTTCACT GGGGTCCATAATTAGCATC 301 58
5a,6 GCTCTCTACAGTAAGTTCTC AGGAGAGAGCATTGGGCTTA 457 59
7 AATCCACCCACTGTCCCG CCAGCCACCTTCATACCG 542 59
8 TCAGGTAACTAACATCGCA GTTGACTGTACTTGGAAGAA 719 53
9,10,11 GAGGTGGGAACCAGTTTGATG CAAGCCAATCTCTGTAGTGCG 890 55
12 GCTGTGTGATGTGTTCCTCA AAGAGAGATCGCCTCTGTG 245 58
13 CATGTCTGTTTCTCAAAGGG CCATAGTCACTGACTGAATTAACAC 202 59

PAX 6, Paired Box 6.