FIGURE 6.

Protein regions that are important for recoverin function. (A) Highly conserved amino acid positions forming the hydrophobic myristoyl pocket. (B) EF hands 2 and 3 of bRec with Ca²⁺-bound (magenta). The essential Glu (E) is indicated in EF-hand 3. An Asn was replaced in silico by Pro (P) to illustrate a possible interference with the position of Glu. Images were created by Pymol using coordinates of Ca²⁺-free and Ca²⁺-bound bRec (PDB codes: 1IKU and 1JSA, Tanaka et al., 1995; Ames et al., 1997).