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. 2018 Sep 28;9:2276. doi: 10.3389/fmicb.2018.02276

Table 1.

Antibacterial spectrum of plantaricin LPL-1.

Indicator strain Source Media Activity (mm) MIC (μg/mL)
Gram-positive bacteria
Listeria monocytogenes 54002 NICPBP TSYEB 16.4 ± 0.23 16
L. monocytogenes 19113(3a) ATCC TSYEB 16.32 ± 0.43 16
L. monocytogenes 19114(4a) ATCC TSYEB 16.21 ± 0.35 16
Staphylococcus aureus 13565 ATCC TSB 9.12 ± 0.42 32
S. aureus 6538 CGNCC TSB 8.21 ± 0.21 32
S. aureus 26112 CVCC TSB 8.31 ± 0.42 32
Enterococcus faecalis M2 Lab MRS 6.32 ± 0.52 32
E. faecalis Lab MRS 7.98 ± 0.11 32
Lactobacillus delbrueckii subsp.lactis Lab MRS 18.81 ± 0.12 16
Lactobacillus plantarum S-35 Lab MRS 17.32 ± 0.33 16
L. plantarum γ-35 Lab MRS 16.98 ± 0.21 16
Lactobacillus bulgaricus Lab MRS 14.32 ± 0.29 16
Lactobacillus salivarius Lab MRS 15.11 ± 0.32 16
Lactococcus lactis NZ9000 Lab MRS 14.43 ± 0.21 16
L. lactis MG1363 Lab MRS 14.12 ± 0.39 16
Bacillus amyloliquefaciens Lab LB 7.43 ± 0.32 32
B. pumilus Lab LB 7.67 ± 0.21 32
Gram-negative bacteria
Escherichia coli DH5α Lab LB 0
E. coli BL21 Lab LB 0
E. coli BW25113 Lab LB 0
E. coli JM109 Lab LB 0
Fungi
Saccharomyces cerevisiae Lab YPD 0
Pichia pastoris GS115 Lab YPD 0

TSYEB media (Tryptone 17 g/L, Soya Peptone 3 g/L, Yeast Extract 6 g/L, Sodium Chloride 5 g/L, Dipotassium Phosphate 2.5 g/L, Glucose 2.5 g/L, and pH 7.3 ± 0.2); TSB media (Tryptone 17 g/L, Soytone 3 g/L, Glucose 2.5 g/L, Sodium Chloride 5 g/L, Dipotassium Phosphate 2.5 g/L, and pH 7.3 ± 0.2); MRS media (Protease peptone 10 g/L, Beef extract 10 g/L, Yeast extract 5 g/L, Glucose 20 g/L, Tween 80 1 g/L, Dipotassium Phosphate 2 g/L, Sodium acetate 5g/L, Ammonium citrate 2 g/L, Magnesium Sulfate 0.2 g/L, Manganese Sulfate 0.05 g/L, and pH 6.5 ± 0.2); LB media (Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L, and pH 7.3 ± 0.2); and YPD media (Yeast Extract 10.0 g/L, Peptone 20 g/L, Glucose 20 g/L, and pH 6.5 ± 0.2).