Table 2.
Purification Stage | Volume (mL) | Total protein (mg) | Total activity (AU) | Specific activity (AU/mg) | Purification fold | Recovery (%) |
---|---|---|---|---|---|---|
Culture supernatant | 1000 | 4,002 | 256,000 | 63.96 | 1 | 100 |
Ammonium sulfate precipitation | 20 | 1,245 | 204,000 | 163.85 | 2.56 | 79.68 |
SP-Sepharose Fast Flow | 10 | 38.7 | 92,000 | 2,377.26 | 37.16 | 35.93 |
Sephadex G10 | 4 | 5.4 | 21,300 | 3,944.44 | 61.66 | 8.32 |
RP-HPLC | 0.3 | 0.96 | 5,320 | 5,541.66 | 86.63 | 2.08 |