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. 2018 Sep 28;9:2276. doi: 10.3389/fmicb.2018.02276

Table 2.

Purification of plantaricin LPL-1.

Purification Stage Volume (mL) Total protein (mg) Total activity (AU) Specific activity (AU/mg) Purification fold Recovery (%)
Culture supernatant 1000 4,002 256,000 63.96 1 100
Ammonium sulfate precipitation 20 1,245 204,000 163.85 2.56 79.68
SP-Sepharose Fast Flow 10 38.7 92,000 2,377.26 37.16 35.93
Sephadex G10 4 5.4 21,300 3,944.44 61.66 8.32
RP-HPLC 0.3 0.96 5,320 5,541.66 86.63 2.08