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. 2018 Aug 20;19(10):e46198. doi: 10.15252/embr.201846198

Figure 3. Transcriptome‐wide analyses of 3′ tRNA cleavage sites by RNA‐Seq.

Figure 3

  1. A complete map of changes in mitochondrial RNA abundance determined by RNA‐Seq coverage (log2 fold change[KOmean/Ctrlmean]) from three control (L/L) and three knockout (L/L, cre) mice, on heavy (outer track) and light (inner track) strands. Increases are shown in red and decreases in blue. The mitochondrial genome is displayed in the central track; rRNAs are displayed in orange, mRNAs in green, tRNAs in blue and the non‐coding region (NCR) in gray. The log fold change scale for the heavy strand is −3 to 8.5 and for the light strand is −3.3 to 3.3.
  2. Genome browser view of the mean RNA‐Seq coverage (log2 fold change[KOmean/Ctrlmean]) showing the 3′ cleavage sites of mt‐tRNA Asp and mt‐tRNA SerAGY by ELAC2. Regions of interests are shown in green (for mRNAs) and blue (for tRNAs) boxes.
  3. Genome browser view of the mean RNA‐Seq coverage (log2 fold change [KOmean/Ctrlmean]) showing the 3′ cleavage sites of a cluster of tRNAs, mt‐tRNA Ile, mt‐tRNA Met, and mt‐tRNA Gln by ELAC2. Regions of interests are shown in green (for mRNAs) and blue (for tRNAs) boxes.
  4. Genome browser view of the mean RNA‐Seq coverage (log2 fold change[KOmean/Ctrlmean]) showing the 3′ cleavage site of mt‐tRNA Glu by ELAC2. Regions of interests are shown in green (for mRNAs) and blue (for tRNAs) boxes.