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. 2018 Aug 20;19(10):e45494. doi: 10.15252/embr.201745494

Figure 1. Gadd45a deletion improves organ maintenance and prolongs the lifespan of G3Terc −/− mice.

Figure 1

  • A, B
    Western blotting analysis of Gadd45a protein expression in mouse intestine crypt lysates from WT and G3Terc −/− mice (A). Quantification of Gadd45a protein expression, β‐actin protein level was used as the loading control (n = 7 mice per genotype), two independent experiments were carried out (B). P‐values were calculated with unpaired two‐tailed Student's t‐test.
  • C
    Quantification of Gadd45a‐ChIP values as percentage of total telomeric DNA and ALU elements in WT and G3Terc −/− MEF cells (n = 3 for each genotype). P‐values were calculated with unpaired two‐tailed Student's t‐test.
  • D
    Dot‐blot assay following Gadd45a‐ChIP in WT and G3Terc −/− MEF cells.
  • E
    Kaplan–Meier survival curves of the mice with indicated genotypes (WT, n = 44; Gadd45a −/−, n = 48; G3Terc −/−, n = 37; G3‐dKO, n = 41). P‐value comparing the survival of G3Terc −/− with G3‐dKO mice was calculated with the log‐rank test.
  • F
    The average body weight of mice with indicated genotypes at indicated time points (G3‐dKO female (n = 19) versus G3Terc −/− female (n = 17), P = 0.004; G3‐dKO male (n = 14) versus G3Terc −/− male (n = 14), P = 0.0038). P‐values were calculated with log‐rank test.
  • G, H
    Whole‐mount images of colon samples from 7‐month‐old mice with indicated genotypes (G). Quantification on density of colon crypts (H) (n = 5 mice per group). P‐values were calculated with unpaired two‐tailed Student's t‐test.
  • I, J
    H&E‐stained small intestine sections from 7‐month‐old mice with indicated genotypes (I). Quantification of the number of small intestine basal crypts (J) (n = 5–6 mice per group). P‐values were calculated with unpaired two‐tailed Student's t‐test.
Data information: Scale bars represent 200 μm in (G) and 50 μm in (I). Data are presented as mean ± SEM.