Figure EV2. Max‐deficient cells generate B220⁺IgM⁺ lymphocytes in vitro .

Sorted GFP ⁺B220⁺IgM⁻ BM cells from HET‐mb1 and MaxKO‐mb1 mice were cultured with IL‐7, rSCF, and rFlt3L and analyzed by flow cytometry 4 days later. For BM analysis, cells were isolated from femora and tibiae and sorted by flow cytometry (GFP ⁺cells, purity > 99%) before culture. After 4 days, the generation of GFP ⁺B220⁺IgM⁺ cells was analyzed. A GFP ⁻ population corresponding to dead cells is indicated. Experiment representative of at least three independent experiments.