FIGURE 5.

Transcriptional profile of key caprine genes over 12 months post challenge with M. paratuberculosis. Panels display quantitative real-time PCR analysis of total RNA extracted from blood samples collected from goat groups at 1 (A), 2 (B), 6 (C), and 12 (D) months post challenge (MPC). Expression levels were calculated with ΔΔCt relative quantitation method relative to the GAPDH gene expression in the naïve group. Target gene names are listed below each panel and fold change for the infected, Mycopar or LAV-vaccinated relative to naïve goat groups are listed on the Y-axis. At each time point, samples from three animals in each group except the infected group at 1 MPC (N = 2) were included and standard errors of the mean (SEM) of the three measurements were presented as error bars.