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Reactive oxygen species level was increased by ET-1 treatment in pulmonary artery smooth muscle cells. (A) Hydrogen peroxide level was increased by ET-1 (cmDCF-DA), (B) superoxide was increased by ET-1 (DHE). *P<0.05, significantly different between C and M groups. C, control group; ET-1, endothelin-1 treatment (1 μM, 24 hours) group.
