Fig. 4.

FACS-based enumeration of myeloid cell infiltration into kidney early in the course of cisplatin-induced AKI. At 2 days following cisplatin, fluorescent cell sorting analysis was performed on single cell suspensions from injured WT and IL-1R1 KO kidneys. A: gating strategy used to parse intrarenal myeloid cell populations after selecting for live CD45⁺ cells and excluding doublets. B–E: total cell numbers for PMN/neutrophils (B), monocytes (C), macrophages (D), and dendritic cells (E), respectively (n = 8 for IL-1R1 WT, n = 7 for IL-1R1 KO). Data are expressed as means ± SE. Significance determined by unpaired t-test.