Fig. 2.

Kidney-specific with no lysine kinase 1 (KS-WNK1) is more potent than long-variant (L)-WNK1. A: thiazide-sensitive ²²Na⁺ uptake in oocytes injected with Na⁺:Cl⁻ cotransporter (NCC) alone or together with human L-WNK1 lacking exon 11 (L-WNK1-Δ11) (solid bars) or KS-WNK1-Δ11 (shaded bars) cRNA. cRNA of L-WNK1-Δ11 and KS-WNK1-Δ11 was injected at a concentration of 0, 1.25, 2.50, or 10.00 ng per oocyte. *P < 0.001; n = 5. B: immunodetection of L-WNK1 and KS-WNK1 (upper blot) and actin (lower blot) from total protein lysate of oocytes injected with 0, 1.25, 2.50, 10.00, or 20.00 ng L-WNK1 or KS-WNK1 cRNA per oocyte. C: functional expression assay shows NCC activity in groups of oocytes injected with NCC cRNA, alone or together with L-WNK1-Δ11 cRNA, KS-WNK1-Δ11 cRNA, or both. For this figure, the level of thiazide-sensitive ²²Na⁺ uptake in the oocytes injected with NCC alone was set to 100%, and the rest of the groups were normalized accordingly. *P < 0.001 vs. NCC; n = 3.