Fig. 3. Exogenous spermidine diminishes oxidative and nitrosative stress during cisplatin nephrotoxicity. The kidneys in C57BL/6 male mice were harvested 1, 2, or 3 days after cisplatin injection or non-injection (control). The mice were orally supplemented with either spermidine (10 mg/kg body weight) or vehicle twice daily from 24 hours before cisplatin injection up to the time that they were sacrificed (n=5 mice in each group). (A) Lipid peroxidation represented by a concentration of lipid hydroperoxide in kidneys. (B) 8-OHdG concentration in kidneys. (C) 8-Nitroguanine concentration in kidneys. ^*P<0.05 vs. control, ^#P<0.05 vs. vehicle.