Fig. 6. Ornithine decarboxylase (ODC) siRNA worsens tubular necrosis and kidney dysfunction during cisplatin nephrotoxicity. The kidneys in C57BL/6 male mice were harvested 1, 2, or 3 days after cisplatin injection or non-injection (control). The mice were orally supplemented with either spermidine (10 mg/kg body weight) or vehicle twice daily from 24 hours before cisplatin injection up to the time that they were sacrificed. ODC siRNA (siODC) or control siRNA (siControl) was injected at 48 and 24 hours before cisplatin injection (n=5 mice in each group). (A) Periodic acid-Schiff stain on kidney sections 3 days after cisplatin injection. Scale bars=50 µm. (B) Tubular necrosis score. (C) Plasma creatinine concentration. *P<0.05 vs. control, #P<0.05 vs. vehicle, †P<0.05 vs. siControl.
