Fig. 4.

Short- and long-axis measurements were consistent in the same mice. Accuracy of each measurement was determined by comparing long-axis volumes with short-axis dimensions. Linear regression analysis showed good correlation for both (A) diastolic (r = 0.89, P < 0.0001) and (B) systolic (r = 0.93, P < 0.0001) measurements, indicating values determined by long axis were in line with changes observed in short axis. EDD, end-diastolic dimensions; EDV, end-diastolic volume; ESD, end-systolic dimensions; ESV, end-systolic volume.