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. 2018 Sep 25;24(13):3455–3465.e5. doi: 10.1016/j.celrep.2018.08.088

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Optogenetic Potentials Evoked by Apical Dendrite Stimulation of Layer 2/3 Pyramidal Neurons Are Reduced in Amplitude during Depolarized Phases of Slow Network Activity

(A) Reconstruction of example layer 2/3 pyramidal neuron showing the soma (black), apical dendrites (green), and basal dendrites (orange), with the apical dendrite two-photon stimulation spot highlighted by cyan arrowhead. Inset shows in vivo image of Alexa-Fluor-594-filled dendrite in red and optogenetic stimulation site in cyan.

(B) Overlaid mean OP_ap from two example cells shows a reduction in amplitude as neurons go from V_hyp (blue) to V_dep (red).

(C) Population average OP_ap shows reduction in amplitude during V_dep.

(D) Amplitude of OP_ap is significantly lower in V_dep compared to V_hyp; gray lines show data from individual cells, filled circles with error bars the mean ± SD.

(E) OP_ap half width is not significantly different between V_hyp and V_dep.

(F) No significant correlation between the ratio of the OP_ap amplitude in V_dep:V_hyp and the OP_ap V_hyp amplitude in awake (purple) and anesthetized (black). Black and purple lines are linear fits.