Figure 5.

Monosynaptic Glutamatergic Input Modulation by Slow Network Activity

(A) Example in vivo two-photon image of two pyramidal neurons stained with Alexa Fluor 594; recording pipettes outlined with white dashed lines; right shows test for monosynaptic connectivity from the same example pair.

(B) Two example, averaged _uEPSPs with different V_hyp (blue) amplitudes; the larger _uEPSP (left) is decreased in V_dep (red) whereas the smaller _uEPSP is increased (right).

(C) Population-averaged, overlaid _uEPSPs during V_hyp and V_dep.

(D) No change in amplitude of _uEPSPs in V_dep as compared to V_hyp across the population. Gray lines show data from individual cells, filled circles with error bars the mean ± SD.

(E) No change in half width of _uEPSPs during V_dep and V_hyp across the entire population.

(F) Significant correlation between log₁₀ of the V_hyp amplitude of _uEPSPs and the ratio of amplitude V_dep:V_hyp, highlighting the amplification of small-amplitude _uEPSPs during V_dep. Correlations performed on the amplitude ratio V_dep:V_hyp and log₁₀ of the V_{hyp u}EPSP amplitude are shown. Open circles represent mean response from a single cell; black line is a single exponential fit.