REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
AAV2/9 pAAV-αCaMKII-hChR2(T159C)-p2A-EYFP	Charité Vector Core	VCA-43a
AAV2/9 pAAV-αCaMKII-hChR2(E123T/T159C)-p2A-EYFP	Charité Vector Core	BA-150a
pLenti-Synapsin-hChR2(H134R)-EYFP	Charité Vector Core	BLV-679
Chemicals, Peptides, and Recombinant Proteins
NaCl	Sigma-Aldrich	S7653
KCl	Sigma-Aldrich	P9333
HEPES	Sigma-Aldrich	H3375
MgCl2∗6H2O	Sigma-Aldrich	M2670
CaCl2∗2H2O	Sigma-Aldrich	C5080
NaOH	Sigma-Aldrich	S8045
Potassium D-gluconate	Sigma-Aldrich	G4500
KCl	Sigma-Aldrich	P9333
Adenosine 5′-triphosphate magnesium salt	Sigma-Aldrich	A9187
Phosphocreatine disodium salt hydrate	Sigma-Aldrich	P7936
Guanosine 5′-triphosphate sodium salt hydrate	Sigma-Aldrich	G8877
HEPES	Sigma-Aldrich	H3375
KOH	Sigma-Aldrich	P5958
Biocytin	Tocris	3349
QX-314 bromide	Tocris	1014
(+)-MK-801 maleate	Tocris	0924
D-890	Abcam	ab120333
Alexa Fluor 594	ThermoFisher	A10438
Urethane	Sigma-Aldrich	U2500
Metamizol	Zentiva	416485
Isoflurane	Cp-pharma	1214
Ketamine 10%	WDT	9089.01.00
Rompun 2% Xylazin	Bayer	KP0CTJS
Vectastain Elite ABC-Peroxidase kit	Biozol	VEC-PK-6100
Denture acrylic	Heraeus	64707963
Agarose, Type III-A	Sigma-Aldrich	A9793
Mowiol	Sigma-Aldrich	81381
Roti-Histofix 4% (PFA)	Roth	P087.4
Sodium phosphate monobasic dihydrate	Sigma-Aldrich	71505
Sodium phosphate monobasic monohydrate	Sigma-Aldrich	S9638
Sodium phosphate dibasic dihydrate	Sigma-Aldrich	71643
Experimental Models: Organisms/Strains
Mouse-C57BL/6J	FEM Charité	C57BL/6J
Mouse-Nex-cre	Klaus Nave	Nex-cre
Mouse-Ai9	The Jackson Lab	007909
Mouse-GAD67-GFP	Yuchio Yanagawa	GAD67-GFP
Mouse-fosGFP	The Jackson Lab	014135
Software and Algorithms
IGORpro 6	Wavemetrics	https://www.wavemetrics.com
MATLAB	MathWorks	https://www.mathworks.com
NEURON	NEURON	https://neuron.yale.edu/neuron
Neurolucida	Microbrightfield	https://www.mbfbioscience.com/neurolucida