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. 2018 Oct 4;16:94. doi: 10.1186/s12958-018-0413-3

Table 1.

Specific primers used for real-time quantitative PCR

Gene Primer Product size (bp) Annealing temperature ©
GAPDH F: AAGCTCATTTCCTGGTATGACAACG 126 61.3
R: TCTTCCTCTTGTGCTCTTGCTGG
PPAR-γ F: ATGACAGACCTCAGACAGATTG 210 54
R: AATGTTGGCAGTGGCTCAG
GLUT-1 F: TATCTGAGCATCGTGGCCAT 238 62.1
R: AAGACGTAGGGACCACACAG
LDLR F: ACTTACGGACAGACAGACAG 223 57
R: GGCCACACATCCCATGATTC

GAPDH glyceraldehyde-3-Phosphate dehydrogenase, GLUT-1 glucose transporter 1, LDLR low-density lipoprotein receptor, PPAR-γ peroxisome proliferator-activated receptor gamma