Fig. 1.

Conservation of Dnmt1, Dnmt3 and Tet in marbled crayfish. Genome annotation revealed the presence of a DNA methylation system consisting of single homologs for Dnmt1 (a), Dnmt3 (b) and Tet (c), respectively. Shown are comparisons of virtually translated protein sequences with three reference organisms: Daphnia pulex, Apis mellifera and Homo sapiens. Numbers in brackets represent accession numbers. Conserved domains are shown as colored boxes. Dnmt1: orange—DMAP1-binding domain, dark blue—replication foci domain, light blue—CXXC zinc finger domain, light green—bromo adjacent homology domain, purple—catalytic domain. Dnmt3: pink—PWWP domain, dark green—zinc finger domain, purple—catalytic domain. Tet: red—catalytic domain, light blue—CXXC zinc finger domain. d Expression of Dnmt1, Dnmt3 and Tet during marbled crayfish development. mRNA expression levels are indicated relative to the TBP (TATA-box-binding protein) housekeeping gene. Bars indicate standard deviations from at least three independent measurements. E: embryonic stages; J: juvenile stages. e mRNA levels of Dnmt1, Dnmt3 and Tet in various adult marbled crayfish tissues (hepatop. hepatopancreas, abd. musc. abdominal musculature)