Table 1.

Oligonucleotide primers and PCR conditions used for amplification of the antibiotic resistance genes and virulence factors in the A. baumannii strains of animal origin [19, 34–46]

Target gene	Primer Sequence (5′-3′)	Size of product (bp)	PCR conditions	Volume (50 μl)
draBC	GCTGGGCAGCAAACTGATAACTCTC CATCAAGCTGTTTGTTCGTCCGCCG	750	1 cycle: 95 0C ------------ 4 min. 30 cycle: 95 0C ------------ 50 s 58 0C ------------ 60 s 72 0C ------------ 45 s 1 cycle: 72 0C ------------ 8 min	5 μL PCR buffer 10X 1.5 mM Mgcl2 200 μM dNTP (Fermentas) 0.5 μM of each primers F & R 1.25 U Taq DNA polymerase (Fermentas) 2.5 μL DNA template
cnf1	AAGATGGAGTTTCCTATGCAGGAG CATTCAGAGTCCTGCCCTCATTATT	498
csgA	ACTCTGACTTGACTATTACC AGATGCAGTCTGGTCAAC	200
cvaC	CACACACAAACGGGAGCTGTT CTTCCCGCAGCATAGTTCCAT	680
iutA	GGCTGGACATCATGGGAACTGG CGTCGGGAACGGGTAGAATCG	300
fyuA	TGATTAACCCCGCGACGGGAA CGCAGTAGGCACGATGTTGTA	880
cnf2	AATCTAATTAAAGAGAAC CATGCTTTGTATATCTA	543	1 cycle: 94 0C ------------ 6 min. 34 cycle: 95 0C ------------ 50 s 58 0C ------------ 70 s 72 0C ------------ 55 s 1 cycle: 72 0C ------------ 10 min	5 μL PCR buffer 10X 2 mM Mgcl2 150 μM dNTP (Fermentas) 0.75 μM of each primers F & R 1.5 U Taq DNA polymerase (Fermentas) 3 μL DNA template
kpsMT II	GCGCATTTGCTGATACTGTTG CATCCAGACGATAAGCATGAGCA	272
PAI	GGACATCCTGTTACAGCGCGCA TCGCCACCAATCACAGCCGAAC	930
papC	GACGGCTGTACTGCAGGGTGTGGCG ATATCCTTTCTGCAGGGATGCAATA	328
fimH	TGCAGAACGGATAAGCCGTGG GCAGTCACCTGCCCTCCGGTA	508	1 cycle: 95 0C ------------ 4 min. 34 cycle: 94 0C ------------ 60 s 56 0C ------------ 45 s 72 0C ------------ 60 s 1 cycle: 72 0C ------------ 10 min	5 μL PCR buffer 10X 2 mM Mgcl2 200 μM dNTP (Fermentas) 0.5 μM of each primers F & R 1.5 U Taq DNA polymerase (Fermentas) 5 μL DNA template
ibeA	AGGCAGGTGTGCGCCGCGTAC TGGTGCTCCGGCAAACCATGC	170
PapG II-III	CTGTAATTACGGAAGTGATTTCTG ACTATCCGGCTCCGGATAAACCAT	1070
sfa/focDE	CTCCGGAGAACTGGGTGCATCTTAC CGGAGGAGTAATTACAAACCTGGCA	410
traT	GGTGTGGTGCGATGAGCACAG CACGGTTCAGCCATCCCTGAG	290
aadA1	TATCCAGCTAAGCGCGAACT ATTTGCCGACTACCTTGGTC	447	1 cycle: 94 0C ------------ 6 min. 33 cycle: 95 0C ------------ 70 s 55 0C ------------ 65 s 72 0C ------------ 90 s 1 cycle: 72 0C ------------ 8 min	5 μL PCR buffer 10X 2 mM Mgcl2 150 μM dNTP (Fermentas) 0. 5 μM of each primers F & R 1.5 U Taq DNA polymerase (Fermentas) 2 μL DNA template
aac(3)-IV	CTTCAGGATGGCAAGTTGGT TCATCTCGTTCTCCGCTCAT	286
sul1	TTCGGCATTCTGAATCTCAC ATGATCTAACCCTCGGTCTC	822
blaSHV	TCGCCTGTGTATTATCTCCC CGCAGATAAATCACCACAATG	768
CITM	TGGCCAGAACTGACAGGCAAA TTTCTCCTGAACGTGGCTGGC	462
cat1	AGTTGCTCAATGTACCTATAACC TTGTAATTCATTAAGCATTCTGCC	547
cmlA	CCGCCACGGTGTTGTTGTTATC CACCTTGCCTGCCCATCATTAG	698
tet(A)	GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA	577
tet(B)	CCTCAGCTTCTCAACGCGTG GCACCTTGCTGATGACTCTT	634
dfrA1	GGAGTGCCAAAGGTGAACAGC GAGGCGAAGTCTTGGGTAAAAAC	367
qnr	GGGTATGGATATTATTGATAAAG CTAATCCGGCAGCACTATTTA	670
imp	GAATAGAATGGTTAACTCTC CCAAACCACTAGGTTATC	188	1 cycle: 95 0C ------------ 4 min. 30 cycle: 95 0C ------------ 45 s 58 0C ------------ 60s 72 0C ------------ 40 s 1 cycle: 72 0C ------------ 5 min	5 μL PCR buffer 10X 1.5 mM Mgcl2 100 μM dNTP (Fermentas) 1 μM of each primers F & R 1 U Taq DNA polymerase (Fermentas) 2.5 μL DNA template
vim	GTTTGGTCGCATATCGCAAC AATGCGCAGCACCAGGATAG	382
sim	GTACAAGGGATTCGGCATCG GTACAAGGGATTCGGCATCG	569
Oxa-23-like	GATCGGATTGGAGAACCAGA ATTTCTGACCGCATTTCCAT	501	1 cycle: 94 0C ------------ 5 min. 32 cycle: 95 0C ------------ 50 s 60 0C ------------ 60 s 72 0C ------------ 70 s 1 cycle: 72 0C ------------ 10 min	5 μL PCR buffer 10X 2.5 mM Mgcl2 200 μM dNTP (Fermentas) 0.5 μM of each primers F & R 1.5 U Taq DNA polymerase (Fermentas) 2 μL DNA template
Oxa-24-like	GGTTAGTTGGCCCCCTTAAA AGTTGAGCGAAAAGGGGATT	246
Oxa-51-like	TAATGCTTTGATCGGCCTTG TGGATTGCACTTCATCTTGG	353
Oxa-58-like	AAGTATTGGGGCTTGTGCTG CCCCTCTGCGCTCTACATAC	599