Fig. 1.
mRNA stability is induced by hypoxic exposure but not treatment with hypoxic mimetics. HEK293T (a-c) or C6 (d) cells exposed to 24 h of Normoxia and either 1% Oxygen (Hypoxia) or the indicated concentrations of DMOG (a), CoCl2 (b), or DFX (c) in the presence 1 g/L glucose before being treated with actinomycin D for 0, 1 or 2 h. mRNA levels of VEFG, MYC, and CYR61 were determined via qPCR, normalized to GAPDH and 0 h time point and half-lives determined. Protein was analyzed via western blotting to confirm treatments had desired effect of HIF induction. Data represents average of N = 3 (HEK293T) or 4 (C6) ± SD for each condition. *P ≤ 0.05 from respective Normoxic (0, Ctrl) condition