Fig. 3.

Hypoxic mRNA stability requires functional mitochondria. HEK293T cells pre-treated with mitochondrial inhibitors rotenone (a) or myxothiazol (b), and then exposed to 24 h of Normoxia or Hypoxia (1% O₂) in the presence of 1 g/L glucose before being treated with actinomycin D for 0, 1 or 2 h. mRNA levels of VEGF, MYC and CYR61 were determined via qPCR, normalized to GAPDH and 0 h time point and half-lives determined. Data represents average of N ≥ 3 ± SD for each condition. *P ≤ 0.05 from respective Normoxic condition; #P ≤ 0.05 from vehicle treated (0) hypoxic sample