Fig. 4.

ROS is necessary for hypoxic mRNA stabilization. HEK293T cells pre-treated with indicated doses of glutathione peroxidase mimetic ebselen and then exposed to 24 h of Normoxia or Hypoxia (1% O₂) in the presence of 1 g/L or 4 g/L glucose before being treated with actinomycin D for 0, 1 or 2 h. mRNA levels of VEGF (a), MYC (b) and CYR61 (c) were determined via qPCR, normalized to GAPDH and 0 h time point and half-lives determined. Data represents average of N = 3 ± SD for each condition. *P ≤ 0.05 from respective normoxic control condition; #P ≤ 0.05; @p ≤ 0.1 from vehicle treated (0) hypoxic sample