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. Author manuscript; available in PMC: 2018 Oct 5.
Published in final edited form as: J Alzheimers Dis. 2017;60(Suppl 1):S133–S150. doi: 10.3233/JAD-170342

Fig. 2.

Fig. 2.

Increased α-Syn expression causes DNA breaks in neurons synergistically with pro-oxidant metals. A, B) Alkaline Comet assay in iFLAG-α-Syn SHSY-5Y cells exposed to 200 μM FeSO4 or CuSO4. α-Syn was induced with Dox for 48 h. Metal salts alone at the same concentration caused only moderate increases in strand breaks. C, D) Semi-quantitative LA-PCR assay for genomic DNA isolated from pCW-iFLAG-α-Syn SHSY-5Y cells in the presence of FeSO4 or CuSO4. ***p ≤ 0.001; ****p ≤ 0.0001.