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Fe-dependent ROS generation and α-Syn aggregation in pCW-iFLAG-α-Syn SHSY-5Y cells. A, B) Cells treated with FeSO4 but not CuSO4 showed a significant increase in nuclear and mitochondrial ROS load. Results are presented as percentage of ROS-positive cells; error bars in the histogram represent the standard error of the mean (SEM) from three independent experiments. C) Immunofluorescence indicates α-Syn aggregate formation in FeSO4-treated cells. **p ≤ 0.01.
