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Phase-contrast photomicrographs of the human and other mammalian cells. HepG2, HH, NIH-3T3, and STO cells at a density of 5 × 105 (A, and B), 2 × 105 (C, and D), 2 × 105 (E, and F), and 2 × 105 cells (G, and H), respectively, were seeded onto 60-mm culture dishes and then cultured for 4 d. The cell storage settings were as follows: −80 °C (A, C, E, and G) and liquid nitrogen (LN2) phase (B, D, F, and H). Scale bar: 200 μm.
