Fig. 4.

Proliferation of HepG2 cells and mammalian cells at −80 °C and in liquid nitrogen (LN₂) phase. HepG2, HH, NIH-3T3, and STO cells at a density of 2 × 10⁵ cells were seeded onto 60-mm culture dishes and then cultured for 4 d. After cell cryopreservation and thawing, the passages were repeated twice. HepG2: HepG2 cells; HH: HH cells; NIH-3T3: NIH-3T3 cells; STO: STO cells. The cell storage settings were as follows: −80 °C (black column) and LN₂ phase (open column). The data represent the means and standard deviation (SD) of three independent experiments.